Figure 5.

Effect of rifampicin on pRNA synthesis and 6S-1 RNA stability. Analysis of 6S-1 RNA steady-state levels following outgrowth from stationary phase by northern blots of cellular RNA extracted at different time points after induction of outgrowth, either in the absence of rifampicin (lanes 2–9) or in the presence of rifampicin (100 μg/ml; lanes 10–17). Lane 1: RNA extracted from stationary cells immediately before 1:5 dilution in fresh LB medium. An antisense 6S-1 RNA, internally labelled with digoxigenin-UTP (top panel), or a 5′-digoxigenin-labelled LNA/DNA mixmer complementary to the pRNA 14-mer (bottom panel) were used as northern probes; for details see Beckmann et al (2010).