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. 2012 Feb 14;31(7):1811–1822. doi: 10.1038/emboj.2012.28

Figure 1.

Figure 1

MBF-dependent transcription is induced in response to DNA replication stress. (A) MBF-regulated transcription is induced in response to HU. Wild-type cells were grown in rich medium, synchronized by α-factor and subsequently released into media without (left panel) or with (right panel) 0.2 M HU. CLN2 (SBF-regulated) and RNR1 (MBF-regulated) RNA from arrested cells (0 min) and cells released from the arrest for the indicated interval was quantitated by RT–qPCR and shown as percentage of the maximal RNA level in untreated cells. (B) MBF-regulated transcription is induced in response to MMS. Wild-type cells were grown as in (A) and released into medium with or without 0.033% MMS. RNR1 RNA was assessed by RT–qPCR and displayed as in (A). (C) Rad53 is required for induction of MBF-regulated transcription in response to DNA replication stress. rad53Δ sml1Δ cells were grown and synchronized as in (A) and subsequently released into media without (left panel) or with (right panel) 0.2 M HU. CLN2 and RNR1 RNA levels were quantitated and displayed as in (A). Budding index is shown as a marker of cell cycle progression.