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. 2012 Feb 21;31(7):1654–1665. doi: 10.1038/emboj.2012.30

Figure 2.

Figure 2

Electrophysiological characterization of whole-cell CatSper currents from human sperm. Currents were recorded at pHi 7.3 in the absence of intracellular divalent ions. The membrane voltage was stepped from −80 to +80 mV in steps of 10 mV from a holding potential of 0 mV. (A) Currents in extracellular solution containing Mg2+ and Ca2+ (HS) and monovalent CatSper currents in divalent-free Na+-based bath solution (NaDVF); perfusion with 80 μM bourgeonal potentiated monovalent currents (NaDVF+Bg). CatSper currents and currents evoked by bourgeonal were completely blocked by 30 μM mibefradil (NaDVF+Bg+Mib). (B) Current–voltage relationship from (A). (C) Currents in solution containing Ca2+ and Mg2+ (HS) and CatSper currents in divalent-free solution (NaDVF). Perfusion with 50 μM undecanal potentiated monovalent currents (NaDVF+Un). Currents were completely blocked by 30 μM mibefradil (NaDVF+Un+Mib). (D) Current–voltage relationship from (C).