Table 1.
Primer | Sequence |
---|---|
GCS-1 | GCGC AGATCT CGTTATGATGAAAATATAATGTTTGG |
GCS-2 | GCGC CTCGAG ATCTTGTGTATTTGAACTATCATTAAC |
GCS-3 | GCGC AGATCT GATGTAATACTTGACAAAAATG |
GCS-4 | GCGC CTCGAG TTATGCACTCAGTTCGTAC |
GCS-5 | GCGC CCGCGG CGGAACGCCATTAAGCTGGGATG |
GCS-6 | GCGC CTTAAG CATGGCCAGTAATTCTTCTAGCC |
GCS-7 | GCGC CCATGG GAGCATCCAAAAGAGAAGCTTTAAC |
GCS-8 | GCGC GGCGCC GCAAGTATTGCGTATAATCCTCTCC |
GCS-9 | CACC ATGGGTTTTCTAAAAATCGGAACG |
GCS-10 | TGCACTCAGTTCGTACATTTTTTTTGC |
GS-1 | GCGC AGATCT GATATGATTGCTTTTTTGAATAC |
GS-2 | GCGC CTCGAG GTCGTTCAAATCGATAAGAAG |
GS-3 | GCGC AGATCT CATGAAGATGATTTTAATAGTTTTG |
GS-4 | GCGC CTCGAG CTCAATGTTCAGTTAAAAAAAAAG |
GS-5 | GCGC CCGCGG GTCCCAATGGAAAGAATGAATATC |
GS-6 | GCGC CTTAAG CGATTAGTCATATAATCAGATCTTCC |
GS-7 | GCGC CCATGG GCCTTACAAGTAGACCCATCTC |
GS-8 | GCGC GGCGCC CCAAGCTTGATATACCACAAATGG |
Restriction sites within the primer sequences used for directional cloning are indicated in italics.