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Comparison between FluoroSpot and ELISpot. Equal numbers of enriched monocytes (1000 or 3000 cells/well) were incubated for 20 h in the presence of lipopolysaccharide (50 ng/ml). The number of cells secreting IL-6, IL-1β, TNF-α, IL-10 and IL-12p40 was determined by the respective method. Similar results were obtained in two separate experiments.
