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. 2012 Feb;75(2):249–258. doi: 10.1111/j.1365-3083.2011.02641.x

Figure 3.

Figure 3

FluoroSpot analysis of IL-6- and IL-1β-secreting monocytes co-secreting TNF-α or MIP-1β. Enriched monocytes (1000 cells/well) were incubated for 20h and analysed for the co-secretion of IL-6/TNF-α, IL-1β/TNF-α, IL-6/MIP-1β, IL-1β/MIP-1β and IL-6/IL-1β in response to lipoteichoic acid (500 ng/ml) or lipopolysaccharide (50 ng/ml). Analysis of each cytokine combination revealed three distinct populations: monocytes secreting either one of the cytokines (green or red bars) or monocytes secreting both cytokines (yellow bars). Values represent the mean ± range of six individuals (n = 6). Pie charts show the average percentage of toll-like receptor-activated monocytes secreting either one of the cytokines or both.