Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Feb;75(2):249–258. doi: 10.1111/j.1365-3083.2011.02641.x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 The Authors. Scandinavian Journal of Immunology © 2011 Blackwell Publishing Ltd

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

PMC Copyright notice

FluoroSpot analysis of IL-6and IL-1β-secreting monocytes co-secreting IL-10 or IL-12p40. Enriched monocytes (3000 cells/well) were incubated for 20 h in FluoroSpot plates and analysed for the co-secretion of IL-6/IL-10, IL-1β/IL-10, IL-6/IL-12p40 and IL-1β/IL-12p40 in response to lipoteichoic acid (500 ng/ml) or lipopolysaccharide (50 ng/ml). Analysis of each cytokine combination revealed three distinct populations: monocytes secreting either one of the cytokines (green or red bars) or monocytes secreting both cytokines (yellow bars). Values represent the mean ± range of six individuals (n = 6). Pie charts show the average percentage of toll-like receptor-activated monocytes secreting either one of the cytokines or both.