Figure 2.
HPLC separation of GSH-TDI reaction products. The absorbance at 210 nm (top) and 245 nm (bottom) was measured (Y-axis) for each 1 min eluent sample collected over time (X-axis). Samples of TDI vapor (<200 ppb) exposed GSH (10mM), shown as blue diamonds, were compared with “mock” exposed GSH, shown as red squares, to identify TDI reaction products, highlighted as peaks A-D, and unreacted GSH (peak ∅).