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. Author manuscript; available in PMC: 2012 Apr 7.
Published in final edited form as: Chem Res Toxicol. 2011 Aug 10;24(10):1686–1693. doi: 10.1021/tx2002433

Figure 4.

Figure 4

Tandem mass spectrometry analysis of peak A from HPLC purified GSH-TDI reaction products. A sample of peak A from the HPLC fractionation of GSH exposed to TDI vapor was analyzed by MS/MS (X-axis = m/z), focusing on the major m/z 456.2 product. The MS/MS spectrum shows a major fragment with an m/z consistent with the Gly-Cys-TDI* structure shown in Figure 6, suggesting linkage of TDI via the thiol of Cys, rather than the glutamate amino acid of GSH. Identical results were obtained with peak B from the HPLC fractionation shown in Fig. 2.