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. 2001 Apr 10;98(9):5353–5358. doi: 10.1073/pnas.061038298

Figure 2.

Figure 2

Purification of ricinosomes on a 44–65% sucrose gradient and Western blot analysis (fraction 1, upper part, and fraction 24, lower part, of the gradient) by using antibodies against the ricinosome marker enzyme CysEP, against the ER-resident BiP (Hsp70) and against marker enzymes for glyoxysomes (gMDH) and mitochondria (mMDH). Fractions 22–24 contain ricinosomes with no contamination by mitochondria or glyoxysomes. Anti-BiP antibodies give a strong signal with the ER in the upper part of the gradient, and a smaller peak in fractions 22–24 containing ricinosomes.