Fig. 1.

Effect of Tat on R28 cell survival and proliferation. a R28 cells were exposed to increasing doses of Tat for 24 and 48 h; 3% H₂O₂ was used as a positive control. Cells were exposed to three concentrations of Tat; 50, 100, 200 ng/ml. Cells were then harvested and analyzed for cell death using flow cytometry. Cell death was not significantly higher on treatment with Tat. Increasing concentrations up to 200 ng/ml did not show significant variation. b R28 cells were treated with increasing doses of Tat for 24 h and 48 h. Cells were then treated with 0.5 mg/ml MTT for 4 h, after which the medium was aspirated, the formazan precipitates dissolved in DMSO, and the amount of formazan conversion analyzed at 570 nm for proliferation/viability. Cell proliferation was not significantly higher on treatment with Tat. Increasing concentrations up to 200 ng/ml did not show variation in results