Table 1.

Acquisition and processing parameters of the experiments shown.

a) 3D 15N-dispersed NOESY: The spectrum was recorded on 0.8 mM sample of the M50 protein (19.4 kDa), sampling density 6% (2,400 of 40,000 complex points), measured on a Bruker Avance 800. FM reconstruction (500 iterations) on a Cuda computer using 4 Nvidia GPU cards was achieved in 1.5 days. IST reconstruction on a 128 cpu cluster was obtained in 3 min.
Dimension	Spectral width (Hz)	Number of complex points	Maximal evolution time (ms)
F1 1H	9,606.148	400	41.62
F2 15N	2,000.00	100	50.00
F3 1H	9,765.6	1024	104.8

b) 4D 13C dispersed methyl-methyl NOESY (150 ms mixing time): The spectrum was recorded on a 1 mM sample of the Med25 complex with the transactivation domain of VP16 (28 kDa), sampling density 14.5%. Recorded on a Bruker Avance 750 with four scans per FID and a total measuring time of 7.5 days. FM reconstruction on one cube took 13 hrs on a Cuda computer using 4 Nvidia GPU cards. IST reconstruction of one cube on a single 32 Xeon cpu required 30 min, and all 339 cubes were reconstructed in 1.5 hrs on a cluster of 128 cpus.
Dimension	Spectral width (Hz)	Number of complex points	Maximal evolution time (ms)
F1 1H	1,650	28	17
F2 13C	3,300	44	13
F3 13C	3,300	96	29
F4 1H	10,000	768	77

c) High-resolution 4D 13C dispersed methyl-methyl NOESY (120 ms mixing time): The spectrum was recorded on a 0.9 mM sample of a 10 kDa protein G construct containing the B1 domain with a sampling density of 0.8% (10,906 of 1,350,000 complex points), measured on a Bruker AMX 500 with four scans per FID and a total measuring time of 5 days. IST reconstruction was achieved in one day on a cluster of 128 cpus.
Dimension	Spectral width (Hz)	Number of complex points	Maximal evolution time (ms)
F1 1H	500.1	60	118
F2 13C	1,257.6	150	118.4
F3 13C	1,257.6	150	118.8
F4 1H	6666.7	1024	154