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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Clin Cancer Res. 2012 Feb 20;18(7):1979–1991. doi: 10.1158/1078-0432.CCR-11-2113

Figure 5. ON 01910.Na overcomes stroma-derived survival signaling.

Figure 5

A, CLL cells (n= 15) were cultured with or without the follicular dendritic cell line HK (ratio CLL:HK 20:1) and viability was assessed by Annexin V-PE in CD19-FITC stained cells by flow cytometry. Differences between groups were assessed by paired Student’s T-test. B, cytotoxic effect of ON 01910.Na at 48 hours on CLL cells in the presence or absence of HK cells measured as in A. C, expression levels of MCL-1, pAKT, and pSAP/JNK in cells from two representative CLL patients treated with ON 01910.Na (2 μM for 24 hours) in the presence or absence of HK cells. D, migration towards SDF-1α (200ng/ml) was assessed for CLL cells pre-incubated with ON 01910 (2 μM) or vehicle (DMSO) for 2 hours. Cell counts were performed in triplicates and the mean(+/− SD) of the migration index normalized to control is shown. Statistical significance was assessed by non paired Student’s T-test