Table 1.
Sequences of PCR primers used in this study.
| Gene | Primer (5′ to 3′) | (Source Species) accession # / reference |
|---|---|---|
| β-actin | Forward: GAC CCA CAC AGT GCC CAT CT | (O. mykiss) AF157514/(Matsuo et al., 2008) |
| Reverse: GTG CCC ATC TCC TGC TCA AA | ||
| GST alpha | Forward: GCT CAC AAG AAG TTT CCG ACA |
(O. mykiss) EZ790503.1; (S. salar) BT046898.1 |
| Reverse: GTC CCC AGG CAC CTT TTA TAC TA | ||
| GST kappa | Forward: CCC AAA CAA GTT CCT GTA TAT GA |
(O. mykiss) BT074032.1; (S. salar) BT043662.1 |
| Reverse: CGT GAC ACC TGC TCT ACC TG | ||
| GST mu | Forward: GAT GAG AAG CAC AAA CTG GG |
(O. mykiss) EZ764534.1 |
| Reverse: AAT CAC AAA GCC GTT CCG GAA G | ||
| GST omega | Forward: TTG GTG GAA ATG CAA TCA CA | (O. mykiss) BT073404.1; (S. salar) BT049799.1 |
| Reverse: GTA AAA TGC CTT GTA GGT CTC CG | ||
| GST pi | Forward: TAT TGT GGG CTA ATG TGT AAG AT |
(O. nerka) AB026119/ (Gallagher et al., 2008) |
| Reverse: CCC TGA AGA GCT TTG TCG | ||
| GST rho | Forward: CTG CTG TGG GGC TCC GGC | (O. mykiss) BT073173.1; (S. salar) BT057296.1 |
| Reverse: ATC TCA AAC ATG CGC TGG | ||
| GST theta | Forward: GGT GAG AAA GGT TCC GGT CA |
(S. salar) BT045588.1 |
| Reverse: GTG TTG CCA AGA CAG GTACTCGT | ||
| GST zeta | Forward: ACA AAG CCC ATT CTT CAT GG | (O. mykiss) EZ805030.1 |
| Reverse: GGC ACT TGT TGC ATA GGG TT | ||
| microsomal GST |
Forward: TAC AGA CAA TTT GCG AAC ATT AT |
(O. mykiss) BT074213; (S. salar) BT048458.2 |
| Reverse: CAC TGA CTA GGC GAG GAC T | ||
| Metallothionein (MT-1A) |
Forward: CAAGTGCTCCAACTGTGCAT | (S. salar) BT059876 |
| Reverse: TACACCAGGCCTCACTGACA | ||
| ribosomal protein L9 |
Forward: AAAAAGCTGCGTGTGGATAAAT | (S. salar) HM4_2302 |
| Reverse: GATCGCATCTTATAGCGGAAAC | ||
| GAPDH | Forward: TCTGTGTTGGAATCAACGGA | (S. salar) BT050071.1; (O. mykiss) AF360980.1 |
| Reverse: TGAAGAAGACTCCGGTGGAC |