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. Author manuscript; available in PMC: 2012 Apr 8.
Published in final edited form as: Methods. 2010 Jun 8;52(2):192–200. doi: 10.1016/j.ymeth.2010.06.010

Figure 3.

Figure 3

Stepwise representation of surface passivation and sample immobilization. (a) The slide surface is cleaned. (b) The slide is aminosilanized and ready for PEGylation. (c) PEG and biotin-PEG molecules are conjugated to the amine-modified surface (d) Streptavidin is bound to the immobilized biotin-PEG molecules. (e) The sample can either be surface-tethered with an attached biotin or indirectly immobilized after being encapsulated in a biotinylated lipid vesicle. After washing to remove unbound sample, the slide is ready for use.