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Effect of overexpression of huMETCAM on tumor formation of a human breast cancer cell line MCF-7 [24]. METCAM-MCF-7 was a clone, which expressed a high level of huMETCAM/MUC18 after transfection with the cDNA. Vector MCF-7 was a clone, which did not express any huMETCAM after transfection with an empty vector. Cells were injected subcutaneously into female SCID mice [24].
