Table 1.
Effect of different time of elution gradient ratio.
| Time (min) | Acetonitrile | 0.03% phosphoric acid aqueous solution |
|---|---|---|
| 0 | 30 | 70 |
| 5 | 30 | 70 |
| 50 | 35 | 65 |
| 60 | 60 | 40 |
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