Table 3.
Effect of AZT (600 mg/kg/d) on the expression levels of genes in the liver of B6C3F1 mice.
| Gene description (gene symbol) | GenBank accession ID | FC | P |
|---|---|---|---|
| Apoptosis (18), P = 0.039 | |||
|
| |||
| Bcl2-associated X protein (Bax) | NM_007527 | 1.143 | 0.020 |
| Bcl2 binding component 3 (Bbc3) | NM_133234 | 1.065 | 0.032 |
| Bcl2/adenovirus E1B 19 k Da-interacting protein 1, NIP3 (Bnip3) | NM_009760 | 1.159 | 0.041 |
|
| |||
| Lipid metabolism (23), P = 0.046 | |||
|
| |||
| Acyl-Coenzyme A synthetase long-chain family member 3 (Acsl3) | AK012088 | 1.166 | 0.001 |
| Acyl-Coenzyme A dehydrogenase, long-chain (Acadl) | NM_007381 | −1.159 | 0.025 |
| Acyl-Coenzyme A dehydrogenase, short-chain (Acads) | NM_007383 | −1.142 | 0.022 |
| Acyl-Coenzyme A dehydrogenase, short/branched chain (Acadsb) | NM_025826 | −1.225 | 0.011 |
|
| |||
| Mitochondrial genes (13), P = NS | |||
|
| |||
| NADH ubiquinone dehydrogenase, subunit 1 (mt-Nd1) | AK018753 | 1.080 | 0.310 |
| NADH ubiquinone dehydrogenase, subunit 2 (mt-Nd2) | AU018363 | 1.080 | 0.280 |
| NADH ubiquinone dehydrogenase, subunit 3 (mt-Nd3) | J01420 | 1.021 | 0.724 |
| NADH ubiquinone dehydrogenase, subunit 4 (mt-Nd4) | AB042809 | 1.018 | 0.827 |
| NADH ubiquinone dehydrogenase, subunit 4L (mt-Nd4l) | AA109866 | 1.048 | 0.526 |
| NADH ubiquinone dehydrogenase, subunit 5 (mt-Nd5) | AU018713 | 1.050 | 0.564 |
| NADH ubiquinone dehydrogenase, subunit 6 (mt-Nd6) | AA435281 | 1.081 | 0.144 |
| Cytochrome b (mt-Cytb) | AU018811 | 1.013 | 0.861 |
| Cytochrome c oxidase, subunit I (mt-Co1) | AU018394 | 1.014 | 0.829 |
| Cytochrome c oxidase, subunit II (mt-Co2) | AU019143 | 1.054 | 0.449 |
| Cytochrome c oxidase, subunit III (mt-Co3) | AB042432 | 1.018 | 0.765 |
| ATP synthase, subunit 6 (mt-ATP6) | AF093677 | 1.008 | 0.906 |
| ATP synthase, subunit 8 (mt-ATP8) | J01420 | 1.067 | 0.464 |
The bold numbers in parentheses indicate number of genes evaluated for the molecular function. P values in bold represent effect of AZT on the molecular function, which is a combined effect of AZT-related changes in expression levels of number of genes evaluated for the molecular function. FC indicates a relative fold change in mice treated with 600 mg/kg/d AZT compared to vehicle-treated control mice. Negative values of fold changes indicate down-regulation of genes. Mitochondrial genes important in energy production, for which 600 mg/kg/d AZT did not alter transcription, are also presented in the table. Sex-related differences in the expression levels of mitochondria-related genes were not observed in liver of AZT-treated mice. NS: Not significant.