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. 2011 Nov 8;20(4):798–807. doi: 10.1038/mt.2011.233

Figure 2.

Figure 2

Presence of corrected stem cells in transduced XP-C keratinocytes cell populations. (a) Western blot analysis of XPC expression in holoclones (C8, C4, and C25) isolated from CD24-XPC-transduced XP798VI keratinocytes (M, corrected mass population). WT1, positive control; XP798VI, parental XP-C keratinocytes. Anti-GAPDH antibody was used as a loading control. (b) Cumulative cell doublings of CD24-XPC-transduced XP798VI keratinocytes (XP798VI+CD24-XPC (mass), open diamonds) and holoclones C4 (black triangles), C8 (black squares), and C25 (black cross). (c) Western blot analysis of XPC expression in CD24-XPC transduced XP798VI keratinocytes (mass) and its derived holoclones C8, C4, and C25 after the indicated population doublings (PD 1–120). (d) Survival of CD24-XPC-transduced XP798VI keratinocytes (mass) and its derived holoclone (clone 4) after exposure to a single UV-solar simulated radiation (UV-SSR, 4,000 J/m2 UVB + 41,300 J/m2 UVA) during long-term serial propagation. Data are represented as mean ± SEM of two independent experiments. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PD, population doublings.