Figure 2.

HIV recovers from long-term RNA interference (RNAi)-mediated inhibition. HIV was propagated in the presence of a (a) Ldr3, TatB2, (b) compartmentalized, or equally distributed RNAi selective pressure for 32 days. In each sample, 4 × 10⁵ cells of a given RNAi composition were infected at a multiplicity of infection (MOI) of 0.0005. Infectious titers were measured every 2 days, and every eighth day viral supernatant was transferred to a new culture with a composition identical to the original, for a total of 32 days. Cultures that exceeded 1,000 IU/ml were deemed to have “escaped.”