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. 2012 Jan 17;20(4):829–839. doi: 10.1038/mt.2011.291

Figure 1.

Figure 1

Intranasal small interfering RNA (siRNA) delivery to the normal rat brain. Fluorescein isothiocyanate (FITC)-labeled transfection indicator siRNAs (2 µg) were administered intranasally. (a) Whole brains were isolated from normal rats and positioned ventral sides upward. (b) Cresyl violet staining of coronal brain sections at the levels indicated in a. (c–p) Localizations of FITC-labeled cells were examined by triple fluorescent staining with FITC, DAPI, and cell-type specific antibodies (anti-NeuN for neuron, anti-GFAP for astrocyte, anti-Iba 1 for microglia) in coronal brain sections prepared at 1, 3, or 12 hours after siRNA administration. (c–j) The superior prefrontal cortex, (k,n) striatum, (l,o) amygdala, and (m,p) hypothalamus are indicated in b as Ct, St, Am, and Hy, respectively. Insets in d–f are high magnification images. (n–p) High magnification images of each brain region (white box) at 3 hours postadministration. (c, g, i, k, l, m) Brain sections from phosphate-buffered saline (PBS)-treated control animals were also included. Arrows indicate transfection indicator siRNA-positive cells. Bar = 50 µm.