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. 2012 Jan 31;20(4):778–787. doi: 10.1038/mt.2011.302

Figure 1.

Figure 1

Adenovirus-encoded UCP (Ad-F-UCP) promotes expression and secretion of VEGF and FGF-2 in cells. (a) Cells were transduced with phosphate-buffered saline (PBS) with or without Ad-F-UCP or Ad encoding β-galactosidase (Ad-LacZ) at multiplicity of infection of 100 (HeLa and MRC-5 cells) or 200 (NIH3T3 cells), and incubated in a serum-free medium. Culture supernatants were saved for enzyme-linked immunosorbent assay (ELISA) and total RNAs were prepared from the cells at 48 hours after transduction. Reverse transcription-polymerase chain reaction (RT-PCR) was performed with total RNAs and relevant primers. The RT-PCR products were separated by a 1% agarose gel electrophoresis, visualized by ethidium bromide, and quantified by densitometry. The band intensity is relatively expressed. (b) ELISA was performed with the culture supernatants three times, each in duplicate. Data are mean ± SD. FGF-2, fibroblast growth factor-2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IGF-2, insulin-like growth factor-2; TGF-β3, transforming growth factor-β3; VEGF, vascular endothelial growth factor.