Figure 3.

GzmK associates with Importin α1. (a) Importin α1 (Impα1) was eluted with the SET complex. The eluted bands were identified by mass spectrometry. (b) GzmK interacts with native importin α1. In all, 10 μg S-AGzmK was incubated with K562 cell lysates followed by co-immunoprecipitation with anti-GzmK antibody (left panel) or anti-importin α1 mAb (right). (c) GzmK binds directly to recombinant importin α1 (rImpα1). In all, 10 μg S-AGzmK and 10 μg rImpα1 were incubated and co-immunoprecipitation assay was performed as described above. IgG served as a negative antibody control, and rGST was used as a negative protein control. IP: immunoprecipitation