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. 2012 Apr 9;3:81. doi: 10.3389/fphys.2012.00081

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Copyright © 2012 Boucher, Gridley and Liaw.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Jag-1/Notch3 signaling modulates VSMC plasticity toward maturation and contraction. Human aortic VSMC were plated on immobilized recombinant rat Jag-1 Fc or Fc control for 48 h to activate Notch receptors. Immunostaining reveals a significant increase in the expression of the contractile protein SM-actin in response to active Notch signaling (A). BrdU incorporation into the nuclei of Jag-1 Fc stimulated cells was significantly reduced in comparison to Fc control plated cells (B). (Green = BrdU positive nuclei, Blue = DAPI for total cell count, n = 15, p < 0.01). Reduction of Notch3 levels via siRNA-mediated knockdown (C) reveals a distinct reduction in endogenous SM-actin levels after 72 h as compared to VSMC receiving a non-targeting control probe (D).