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Expression of apoptosis related proteins in cells treated with curcumin-ND. Granta cells were incubated with empty-ND or curcumin-ND for specified times. Following incubation, cell homogenates were separated by SDS-PAGE, transferred to nitrocellulose, and probed with antibodies directed against caspase-9, caspase-3, PARP, and GAPDH (A), pIκBα, IκBα, and GAPDH (B), FoxO3a, p27, and GAPDH (C), pAkt, Akt, Bcl2, and GAPDH (D), and cyclin D1 and GAPDH (E).
