Genital tracts of animals pre-treated with either PBS, control diabody (A10), or anti-EMP2 diabody (KS83) were removed at day 4 post-infection. Tissues homogenates were plated on monolayer McCoy cells, and inclusion bodies were detected by immunofluorescent staining of Chlamydia LPS for the determination of bacterial load recovery (IFU). A significant decrease in chlamydial infectivity was observed in KS83 treated animals, and the reductive effect was more prominent in the upper genital tract. Statistical analysis of the IFU measurements were performed using a Student’s t-test (*p-value<0.05, **p-value<0.005, ***p-value<0.0001). Data are expressed as the mean ± SEM from two independent experiments, with n=7 for each experiment.