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. 2012 Jun 5;367(1595):1542–1551. doi: 10.1098/rstb.2011.0238

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Figure 3. — Homo- and hetero-interaction of RTCS and RTCL. (a) Yeast-two-hybrid interaction of RTCS and RTCL proteins fused to a GAL4 activation domain (AD: red) and a Gal4 DNA-binding domain (BD: blue). LOB domains are highlighted in green, full-length variable regions are coloured in yellow. Truncated variable regions without the activation domain are marked in orange. The interaction between the pGBKT7 (BD)-p53 and pGADT7 (AD)-SV40 large T-antigen was used as a positive control, and the interaction between pGBKT7(BD)-lam and pGADT7(AD)-SV40 as a negative control. (b) Interaction of RTCS and RTCL LOB domains and full-length proteins in the bimolecular fluorescence complementation (BiFC) system in Arabidopsis thaliana protoplasts. BiFC was quantified by flow cytometry.