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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Apr 29;1808(9):2147–2155. doi: 10.1016/j.bbamem.2011.04.009

Figure 8. Oxidation of TLCL by cyt c.

Figure 8

Cyt c (5 μM) was incubated with liposomes (with total of 250 μM phospholipids) containing 20% TLCL, in the presence of H2O2 (100 μM) in 20 mM HEPES, pH 7.4, containing 100 μM DTPA for 10 min at 37 °C. At the end of incubation lipids were extracted and resolved by LC-ESI-MS.

A. - Typical ESI-LC-MS spectra of TLCL incubated in the presence of wild type cyt c.

B. - Accumulation of hydroperoxy- (a) and hydroxy- (b) molecular species of TLCL formed in the presence of either wild-type cyt c or different cyt c mutants. Data are expressed as means ± SD, n =4 for wild type and n= 6 for cyt c mutants, *p<0.03 vs. wild-type cyt c. wt-wild type, Y48F-Tyr48Phe, Y67F-Tyr67Phe, Y74F-Tyr74Phe, Y97F-Tyr97Phe.