Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 May;17(5):903–906. doi: 10.3201/eid1705.101569

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

PMC Copyright notice

Phylogenetic trees of West Nile virus strains isolated during outbreaks in Italy, 2008–2009, based on nucleotide sequences of the complete envelope gene. Phylogenetic tree and distance matrices were constructed by using nucleotide alignment, the Kimura 2-parameter algorithm, and the neighbor-joining method implemented in MEGA version 4.1 (www.megasoftware.net/mega4/mega41.html). The tree was rooted by using Usutu virus as the outgroup virus. The robustness of branching patterns was tested by 1,000 bootstrap pseudoreplications. The percentage of successful bootstrap replicates is indicated at nodes, showing only values >80. The scale bar indicates nucleotide substitutions per site. Strains sequenced in this study are TOS-09, FAN-09, BAL-09, CHI-09, NAL-09, and MAN-08. Envelope nucleotide sequence GenBank accession nos: NAL-09, HM991272; TOS-09, HM991273; BAL-09, HM991274; CHI-09, HM991275; MAN-09, HM991276; FAN-09, HM991277. Boldface indicates the 6 newly sequenced strains.