Table A1. Comparison of amino acid substitutions of West Nile virus strains isolated in Italy, 2008–2009, to different European and Mediterranean West Nile virus strains and to reference strain EG101*.
| AA position in WNV polyprotein | AA position in WNV protein | TOS‐09 | NAL‐09 | CHI‐09 | BAL‐09 | FAN‐09 | MAN‐08 | Italy 15803/08 (FJ483548) | Italy 15217/08 (FJ483549) | Italy‐09 (GU011992) | Italy/98 (AF404757) | Morocco/03 (AY701413) | France 405/04 (DQ786572) | Spain GE2o/V (FJ766332) | Portugal/04 (AJ965626) | EG101 (AF260968) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 341 | E‐51 | A | A | A | A | A | A | A | A | A | A | A | A | T | A | A |
| 378 | E‐88 | P | P | P | P | P | P | P | P | P | P | P | P | S | P | P |
| 443 | E‐153 | G | G | G | G | G | G | G | G | G | G | G | R | G | G | G |
| 602 | E‐312 | L | L | L | L | L | L | L | L | L | L | F | L | L | L | L |
| 1754 | NS3‐249 | T | T | T | P | P | P | P | P | P | T | T | T | P | P | P |
| 2209 | NS4a‐85 | I | I | I | I | I | I | I | I | I | V | V | V | V | V | V |
| 2224 | NS4a‐100 | S | S | S | S | S | S | S | S | S | P | P | P | P | P | P |
| 2265 | NS4a‐141 | M | M | M | L | M | M | M | M | M | M | M | M | M | M | L |
| 2359 | NS4b‐86 | F | I | F | F | F | F | F | F | F | F | F | F | F | F | F |
| 2516 | NS4b‐243 | I | I | I | T | T | T | T | T | T | T | T | T | T | T | T |
| 2786 | NS5‐258 | A | A | A | A | A | A | A | A | A | V | V | V | V | V | V |
| 2920 | NS5‐292 | P | P | P | P | S | S | S | S | S | S | S | S | S | S | R |
| 2950 | NS5‐422 | K | K | K | K | K | K | K | K | K | R | R | R | R | R | R |
| 3169 | NS5‐641 | T | T | T | T | A | A | T | T | T | T | T | T | T | T | T |
| 3407 | NS5‐879 | I | I | I | H | I | I | I | I | I | I | I | I | I | I | I |
*The amino acid position numbers refer to the EG101 strain. GenBank accession numbers are shown in parentheses. WNV, West Nile virus; E, envelope; NS, nonstructural.