Western immunoblots showing clear atypical scrapie profiles in sheep in
the following brain regions; brainstem of donor ARRa (lane 7), frontal
cortex of donor ARRb (lane 8) and frontal cortex of donor AHQ (lane 19).
The hippocampus and basal nuclei of recipient animal 11 (lanes 15 and
16, respectively) and cerebellum of recipient animal 12 (lane 18). No
discernible signal was seen in the medulla of donor ARRb (lane 9), and
only a faint profile was visible for the obex of recipient animal 12
(lane 17). Lane 1, animal 2 obex; lane 2, animal 1 obex; lane 3, animal
3 obex; lane 4, animal 4 obex; lane 5, animal 5 obex; lane 6, animal 6
obex ; lane 7, donor ARRa rostral B.stem; lane 8, donor ARRb frontal
cortex; lane 9, donor ARRb caudal medulla; lane 10, animal 7 obex; lane
11, animal 8 obex; lane 12, animal 9 obex; lane 13, animal 10 obex; lane
14, case 11 obex; lane 15, animal 11 hippocampus; lane 1, animal 11
basal nuclei; lane 17, animal 12 obex; lane 18, animal 12 cerebellum;
lane 19, donor AHQ frontal cortex; AS, atypical scrapie; BSE, classical
bovine spongiform encephalopathy; Sc, classical scrapie; MM, molecular
mass marker.