Appendix. Table. Immunohistochemical/ELISA results for sheep infected with scrapie for each tissue*.
Genotype |
ARR/ARR |
AHQ/AHQ |
|||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Months
postinoculation |
12 |
24 |
12 |
24 |
|||||||||||
Donor
inoculum |
ARRa |
ARRa |
ARRa |
ARRa |
ARRa |
ARRb |
AHQ |
AHQ |
AHQ |
AHQ |
AHQ |
AHQ |
|||
Animal no. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |||
Clinical status | Norm | Norm | Inc | Norm | Norm | Inc | Norm | Norm | Norm | Inc | Norm | Suspect | |||
Obex | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Cerebellum† | −/−† | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/−‡ | +/+‡ | |||
Rostral medulla | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Caudal midbrain | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Rostral midbrain | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Thalamus | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | +/−‡§ | +/+ | |||
Occipital cortex | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Parietal cortex | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Frontal cortex | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | +/+‡ | +/+ | |||
Spinal cord C5 | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Spinal cord T1 | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Spinal cord T6 | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Spinal cord T12 | −/− | −/− | –/ITT | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |||
Spinal cord L5–6 | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Palatine tonsil | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | |||
Spleen† | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |||
Mesenteric lymph node | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |||
Distal ileum† | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/−‡ | −/−‡ | −/− | −/− | −/−‡ | |||
RAMALT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
Coeliaco-mesenteric plexus | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | ITT/NT | –/NT | –/NT | –/NT | |||
Extraocular muscles | –/ITT | –/ITT | –/ITT | −/− | –/ITT | −/− | –/ITT | −/− | −/− | −/− | −/− | −/− | |||
Medial retropharyngeal lymph node | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
Submandibular lymph node | NT/ITT | NT/ITT | NT/ITT | NT/ITT | NT/– | NT/– | NT/– | NT/ITT | NT/ITT | NT/– | /ITT | −/− | |||
Submandibular salivary gland | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
Cervical thymus | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
Prescapular lymph node | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
Kidney | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
Liver | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
Buffy coat | NT/– | NT/– | NT/– | NT/ITT | NT/ITT | NT/ITT | NT/– | NT/– | NT/– | NT/ITT | NT/ITT | NT/ITT |
*ARR/ARR, Prp genotype of 6 Poll Dorset lambs; AHQ/AHQ, PrP genotype of 6 Cheviot lambs; mpi, months post inoculation; Norm, normal; Inc, inconclusive; Suspect, suspected; NT, not tested; ITT, insufficient tissue to test; RAMALT, recto-anal mucosa–associated lymphoid tissue; PrPSc or protease-resistant form present (+) or absent (–). †Tissues being investigated by bioassay in Tg338 mice. ‡Tissues are positive in bioassay. §High negative result in ELISA.