Figure 4.
Assessment of LPC to restore ANCA induced exocytosis in DGK-inhibited neutrophils. Primed neutrophils (2.5 × 105) in the presence of cytochalasin B were treated with ANCA IgG (200 μg/mL) or IgG (200 μg/mL) from healthy control donors for 15 min in the presence or absence of LPC (250 nmol/L). Paired samples of cells were treated with R59022 (18 μmol/L) for 10 min before priming. Supernatants were collected and analyzed for the activity of MPO using a specific substrate. Data have been normalized as a percentage of the unstimulated (unstim) uninhibited control cells. Data are the mean of six different neutrophil donor responses using a panel of two normal IgG preparations and three ANCA preparations (one MPO-ANCA, two PR3-ANCA). *P < 0.05 by one-way ANOVA with posttest.