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. 2010 Jul;16(7):1158–1161. doi: 10.3201/eid1607.091682

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Figure A1 — Recombination analyses of Saffold cardioviuses (SAFVs), isolated in Beijing, China, 2007–2009. The relationships among the aligned cardiovirus genome sequences were analyzed by SimPlot software version 3.5.1 (11). A) Sliding-window SimPlot graph generated by using the sequence of the BCHU79 (SAFV-2) as a query against SAFVs, Theiler-like virus, and Theiler murine encephalomyelitis virus–GDVII. B) Bootscan analysis of the sequence of SAFV BCHU79 in comparison to SAFVs, Theiler-like virus, and Theiler murine encephalomyelitis virus–GDVII. C) Sliding-window SimPlot graph generated by using the sequence of the BCH1031 (SAFV-3) as a query against SAFVs, Theiler-like virus, and Theiler murine encephalomyelitis virus–GDVII. D) Bootscan analysis of the sequence of SAFV BCH1031 in comparison to SAFVs, Theiler-like virus, and Theiler murine encephalomyelitis virus–GDVII. Because the genome sequence identity between BCH1031 and BCH115 is as high as 94%, BCH1031 was used to conduct the SimPlot analysis. The nucleotide position is given on the x-axis, and the percent nucleotide sequence similarity is given on the y-axis. The positions relative to viral genes are shown by genome diagrams above the plots and scans. SAFV-1 (prototype), SAFV-2 UC1, SAFV-2 D/VI/2229/2004, SAFV-2 BR118/2006, SAFV-3 D/VI/2273/2004, SAFV-3 D/VI/2223/2004, SAFV-3 NL2007, SAFV-5 Pak5003, SAFV-5 Pak5152, SAFV-6 Pak 6572, Theiler-like virus NGS910, and Theiler murine encephalomyelitis virus–GDVII were used as reference sequences (GenBank accession nos. NC009448, NC010810, EU681176–EU681179, FM207487, FJ463615–FJ463617, AB090161, and X56019).