Table. Selected inner primers and probe used to amplify genes from cerebrospinal fluid of patient with subacute meningitis, Sweden, 2006*.
| Gene | Primers and probe | Nucleotide sequences, 5′ → 3′ | Product size, bp | |
|---|---|---|---|---|
|
ompB
|
ompB-IF |
CCAATGGCAGGACTTAGCTACT |
267 |
|
|
|
ompB-IR |
AGGCTGGCTGATACACGGAGTAA |
|
|
| 17 kDa |
RH 17-IF |
GCATTACTTGGTTCTCAATTGG |
214 |
|
|
|
RH 17-IR |
AACCGTAATTGCCGTTATCCGG |
|
|
| 16SrDNA |
Ric-F |
TCTAGAACGAACGCTATCGGTAT |
757 |
|
|
|
Ric-R |
TTTCATCGTTTAACGGCGTGGACT |
|
|
|
gltA |
SFG-CS-F |
TGCCAAATGTTCACGGTACTTT |
74 |
|
|
|
SFG-CS-R |
CACAATGGAAAGAAATGCACGA |
|
|
| SFG-CS-Probe | TGCAATAGCAAGAACCGTAGGCTGGATG | |||
*ompB, outer membrane protein B; gtlA, citrate synthase.