Table 2. Oligonucleotide sequences for primers and probes and dye labels used in confirmatory molecular testing for pandemic (H1N1) 2009 virus, National Veterinary Services Laboratories, Ames, Iowa, USA, 2009*.
| Name | Sequence (5′ → 3′) | Description |
|---|---|---|
| Influenza A M screening assay | ||
| M+25 | AGATGAGTCTTCTAACCGAGGTCG | AI M forward primer |
| M-124 | TGCAAAAACATCTTCAAGTCTCTG | AI M reverse primer |
| M-124siv | TGCAAAGACACTTTCCAGTCTCTG | H1N1 M reverse primer |
| M+64 | TCAGGCCCCCTCAAAGCCGA | M probe, BHQ, FAM |
|---|---|---|
| Pandemic influenza N1 differentiation assay | ||
| N1 220F | CAACACCAACTTTGCTGC | N1 forward primer |
| N1 330R | GGAACCGATTCTTACACTGTTGTC | N1 reverse primer |
| N1 232 | CAGTCAGTGGTTTCCGTGAAATTAGC | N1 BHQ, FAM |
*Primers and probes were obtained from Integrated DNA Technologies (Coralville, IA, USA) and Biosearch Technologies, Inc. (Novato, CA, USA), respectively. M, matrix; AI, avian influenza; N, neuraminidase.