Table 1. Effect of initial cell concentration and incubation time on sensitivity of the method.

	Incubation time (min)	Cell suspension volume (OD600 nm∼1.5) added
		5 µl	10 µl	20 µl
linear range (µg l−1 glucose C)		0–400	0–500	0–800
	30	0.0083	0.0114	0.0135
	60	0.0071	0.0059	0.0052
	90	0.0037	0.0013	0.0022
	120	0.0016	0.0007	0.0014