Table 1. Primer sets for the pluripotent genes, hematopoietic commitment genes by RT-PCR.
Genes | Sequence (5′ to 3′) F | Sequence (5′ to 3′) R |
Oct4 | TTCAGCCAAACGACCATC | GGAAAGGGACCGAGGAGTA |
Sox2 | AAACAGCCCGGACCGCGTCAA | TCGCAGCCGCTTAGCCTCGT |
Nanog | CCTATGCCTGTGATTTG | AGAAGTGGGTTGTTTGC |
Rex1 | GGCAAAGACAAGACACC | GCAAATTCTGCGAGCT |
GAPDH | AAGGTCGGAGTCAACGG | GGAAGATGGTGATGGGATT |
Brachyury | TGAGCCTCGAATCCACAT | GGGCACCTCCAAACTGA |
GATA-2 | GGCGTCAAGTACCAGGTGT | GGTCGGTTCTGCCCATTC |
SCL | ATGCCTTCCCTATGTTCACCA CCA | TGAAGATAGCCGCACAACTT TGG |
TAL-1 | TTGTGCGGCGTATCTTC | CAGGGTCCTTGCCAGTC |