Table 1. Comparison of disease signs in the prairie dog challenge study.

Study and disease characteristics	USA 2003		USA+CCP		ROC 2003		ROCΔCCP
	n = 4*		n = 4		n = 4		n = 4
Primary lesion onset	P2	–	P7	Day 8	P8	Day 8	P5	Day 10
	P4	Day 10	P12	Day 10	P9	Day 8	P11	Day 10
	P6	Day 10	P14	Day 10	P10	Day 8	P13	Day 10
	P16	Day 10	P15	Day 10	P18	Day 8	P17	Day 10
Secondary/generalized lesion onset †	P2	–	P7	Day 14	P8	Day 10	P5	Day 14
	P4	Day 14	P12	Day 12	P9	Day 12	P11	Day 12
	P6	Day 12	P14	Day 14	P10	Day 12	P13	Day 12
	P16	Day 12	P15	Day 14	P18	Day 10	P17	Day 10
Day 14 secondary lesion count	P2	–	P7	6	P8	15	P5	9
	P4	5	P12	8	P9	13	P11	11
	P6	8	P14	3	P10	14	P13	10
	P16	7	P15	7	P18	15	P17	12
Objective/subjective signs of disease	Crusty nose, labored breathing, bloody oral swabs, inappetence, lethargy, weight loss		Crusty nose, labored breathing, bloody oral swabs, inappetence, lethargy, weight loss		Crusty nose, ocular lesions, bloody oral swabs, inappetence, lethargy, severe weight loss, labored breathing		Crusty nose, ocular lesion, bloody oral swabs, inappetence, lethargy
Mortality	50%		50%		100%		0%
Day of death/euthanasia (PD#)	10 (PD2), 14 (PD4)		18 (PD12, PD15)		12 (PD18), 14 (PD10), 16 (PD8, PD9)		–
MPXV DNA detected in oral swabs	Days 2–30		Days 4–26		Days 2 – death		Days 4–30
MPXV viable virus in oral swabs	Days 2–20		Days 4–20		Days 2 – death		Days 4–22
Anti-OPXV antibodies	All surviving animals anti-OPXV positive		All surviving animals anti-OPXV positive		All animals anti-OPXV positive		All animals anti-OPXV positive

^*- Prairie dog PD2 (underlined) died post-anesthesia on day 10.

^†- Prairie dogs were observed every other day.