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. 2012 Apr 9;7(4):e35054. doi: 10.1371/journal.pone.0035054

Figure 1. JCV T-antigen is downregulated by glucose deprivation in vitro.

Figure 1

Endogenous JCV T-antigen expression was monitored in the cell lines, BsB8 (A and B) and HJC-2 (C and D), after 24-hour glucose deprivation by both western blot and immunocytochemical detection of T-antigen. E. Expression of T-antigen during conditions of glucose deprivation in the above cell lines was quantified. BsB8 cells were exposed to glucose deprivation for 24 hours and were then exposed to a subsequent time-course of normal medium containing 1 g/L D-glucose, after which T-antigen expression was measured by western blot (F) and immunocytochemistry (G). C, control; GD, glucose deprivation.