Skip to main content
. 2012 Apr 9;7(4):e35054. doi: 10.1371/journal.pone.0035054

Figure 2. T-antigen and AMPK exhibit reciprocal repression during glucose deprivation.

Figure 2

A. BsB8 cells were treated with glucose deprivation for 24 hours, and ATP levels were quantified. Results are presented as the concentration of ATP and are normalized to the total protein content of each sample. The experiment was conducted in triplicate. B. BsB8 cells were treated with the indicated doses of the AMPK activating agent, AICAR, for 24 hours, and T-antigen expression was measured by western blot. C. Quantification of T-antigen expression in B. (*, p<0.05). D. BsB8 cells were treated with fenofibrate at the indicated doses for 24 hours, and T-antigen and phosphorylated AMPK levels were determined by western blot. E. BsB8 cells were treated with various doses of the inhibitor of AMPK activation, Compound C, or DMSO, under conditions of glucose deprivation for 24 hours, and T-antigen expression was measured by western blot. F. Quantification of T-antigen expression in E. G. Bs1a, Bs1f, and BsB8 cells were exposed to glucose deprivation for 24 hours, and T-antigen expression and AMPK phosphorylation were measured by western blot. H. U-87MG cells that were transduced with adenovirus expressing T-antigen, Null, or were non-transduced were exposed to glucose deprivation for 24 hours, and T-antigen and phosphorylated AMPK levels were measured by western blot. C, control; GD, glucose deprivation.