Figure 3. T-antigen downregulation during glucose deprivation is associated with AMPK-mediated cell cycle control.

A. BsB8 cells were exposed to glucose deprivation for 24 hours, and the expression of T-antigen and cell cycle regulators was monitored by western blot. B. BsB8 cells were exposed to the indicated doses of AICAR, and the expression of T-antigen and cell cycle regulators was measured by western blot. C. BsB8 cells were treated with 5 uM Compound C (CC) or DMSO (D) and exposed to glucose deprivation for 24 hours, and the expression of cell cycle regulators was monitored by western blot. D. Cell cycle analysis using Guava cell cycle reagent was performed in cells from C. (*, p<0.05). E. BsB8 cells were transfected with CMV-cyclin E or empty vector, and T-antigen expression was monitored by western blot. C, control; GD, glucose deprivation.