Figure 4. T-antigen prevents G1 arrest and preferentially arrests cells in the G2 phase in glucose-deprived cells.

A. Bs1a, Bs1f, and BsB8 cells were treated with glucose deprivation or control medium for 16 hours and were subsequently processed for whole-cell protein extraction for western blot analysis of cell cycle regulatory proteins. B. Identical samples from A. were processed for cell cycle analysis using Guava cell cycle reagent. (* = control compared to GD, p<0.05; # = BsB8 compared to Bs1a and Bs1f cells, p<0.05). C, control; GD, glucose deprivation.