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. 2012 Apr 9;7(4):e34797. doi: 10.1371/journal.pone.0034797

Figure 3. Nonaplex scheme of ITS2 species-diagnostic primers.

Figure 3

Representation of the shark nuclear 5.8S and 28S ribosomal RNA genes and ITS2 locus showing relative annealing sites and orientation of primers used in the nonaplex-PCR assay. Shark universal primers (Fish 5.8SF and Fish 28SR) are shown as gray irregular pentagons, while the seven sharpnose species-specific primers are shown by dark gray irregular pentagons. Rlal: Rhizoprionodon lalandei; Rtay: R. taylori; Rolig: R. oligolinx; Rter: R. terranovae; Rlong: R. longurio; Rpor: R. porosus; Racut: R. acutus.