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. 2012 Apr 9;7(4):e34797. doi: 10.1371/journal.pone.0034797

Figure 5. Nonaplex primer amplification gel profile.

Figure 5

Results of amplification reactions utilizing the nine-primer nonaplex: two shark universal primers and all the seven species-specific primers. Lanes 1–7 show nonaplex-PCR amplification products of target Rhizoprionodon samples: 1- R. acutus; 2- R. porosus; 3- R. longurio; 4- R. terranovae; 5- R. oligolinx; 6- R. taylori; 7- R. lalandei. Lanes 8–17 show nonaplex-PCR amplification products from non-target species: 8- Galeocerdo cuvier; 9- Negaprion acutidens; 10- Carcharhinus porosus; 11- Prionace glauca; 12- Isurus paucus; 13- Alopias superciliosus; 14- Squalus acantias; 15- Nebrius ferrugineus; 16- Squatina californica; 17- Hexanchus griseus. Lines 8–11, Carcharhiniformes; Line 12, Orectolobiformes; Line 13, Squaliformes; Lines 14–15, Lamniformes; Line 16, Squatiformes; Line 17, Hexanchiformes; Line 18 is the negative control. Lanes labeled “M” contain the molecular size-standard 1kb plus. Faint non-specific bands likely correspond to pseudogenes or uncommon variant copies of ribosomal genes rarely amplified by universal and species-specific primers.