Table 4. Species-specific primers designed along with their sequences and expected amplicon sizes for each species.

Species-specific primers	Primer sequences	PCR product sizes (bp)
Racut-555 ITS2	5′ TTAACGTTCTGTGCGTGTCGAGT3′	230 pb
Rpor-1260 ITS2	5′GCGAGGCACACCTCGGCAC3′	420 pb
Rlong-1116 ITS2	5′GACTTGCTCTGTCCTTGAGCCC3′	560 pb
Rter-946 ITS2	5′TGTGAATAGGGGCAGCCGACA3′	720 pb
Rolig-741 ITS2	5′TACCGGGAGAGCTCGGAAAACGT3′	850 pb
Rtay-482 ITS2	5′AACGGTTCGGGTGCTCCGGCA3′	1150 pb
Rlal-293 ITS2	5′GGCACGTAGGCACCGCCCGCTAT3′	1300 pb

Rlal: Rhizoprionodon lalandei; Rtay: R. taylori; Rolig: R. oligolinx; Rter: R. terranovae; Rlong: R. longurio; Rpor: R. porosus; Racut: R. acutus.