Figure 4. Lateral mobility of cortical ER determines the positioning of endocytic events.

Initiation of endocytic events in cells co-expressing Ede1-GFP and ss-dsRed-HDEL was monitored in a time-lapse experiment (20 frames, 30 s/frame). Superposition of all the frames is presented. The 4^th column represents the superimposed binarized ER signals from 20 consecutive frames in a false-color blue-to-red scale to highlight the dynamics of the cortical ER network (see Fig. 3 legend for an explanation). The column on the far right shows this superposition of binarized ER signals in red overlaid by the green channel, in which the positions of the maxima of the Ede1 sites in the 20 frames were marked by round spots. Bar: 1 µm.