| ATTGGCCCCAGGAAACCCCAGCCTGTCAGCACCTGTTCCAGGATCCAGTTCCCAGCGCAGTATGgatatctccaacctgctg (forward)GAGCCAGGACAGCCAGCAGAATCAGGATCAGGGGCAAGGTCTGGACAGGCAGTGTGGATGCgctagccgttaattaagaag (reverse)Using pIndu-perfect as a template to get a 3498 bp iCreERT2 Cassette. Capital letters are from Upk2 sequence, lower-case letters are from iCreERT2 sequence. |
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CTGAGGCTACAGTGCCCAAG (forward)GTCTAGCGCTCTGAAACCCTC (reverse)Using modified BAC DNA as a template to get a 3772 bp (or 274 bp for unmodified BAC) product. |
| cttatcgatgataagctgtcaaacatgagaattgatccggaacccttaatTCTTAGACGTCAGGTGGCAC (forward)ccgatgcaagtgtgtcgctgtcgacggtgaccctatagtcgagggacctaTCACGTTAAGGGATTTTGGT (reverse)Using pTAMP as a template to get a 860 bp Amp cassette to delete the loxp site in BACe3.6. Lower-case letters are from BAC3.6 sequence, capital letters are from Amp sequence. |
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ATGGATATCTCCAACCTGCTG (forward)AGATCTCCTGTGCAGCATG (reverse)Using tail genomic DNA to get a 312 bp product for TgUICBAC transgenic mice PCR genotyping. |
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TGCTCCCAGGTGGTGGATCTC (forward)CTCAAAGCGGACCTCCTGTTC (reverse)Using BAC DNA as a template to get a 389 bp probe for Southern blot genotyping. Using the probe and ScaI digestion to get 8869 bp and 11196 bp for transgenic and Wt allele on Sothern blot, respectively. |
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CGCGAGAGGAGTGTGTCTG (forward)CTCTGAATCGAGGGATGGAGT (reverse)qRT-PCR for Bcl9l
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AGGCCGAGCAGAAACTTGC (forward)GGCTTAATGTCTGGACCATCTTT (reverse)qRT-PCR for Foxr1
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TGGCCTTCTACAGTAACAGCA (forward)GCATGAATACCGCCTTAAAGGAC (reverse)qRT-PCR for Cxcr5
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AGGTCGGTGTGAACGGATTTG (forward)GGGGTCGTTGATGGCAACA (reverse)qRT-PCR for Gapdh
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