Figure 4. The loss of MEF2A/D does not alter basal synaptic transmission.

(A) mEPSC frequency is unchanged upon deletion of Mef2a/d in hippocampal culture neurons using lentivirus expressing Cre recombinase (p>0.05). The number of recordings is shown in the bar graph for the GFP and Cre infected neurons. Data is shown as mean ± SEM. (B) Lentivirus containing either GFP or Cre was infected at 4 DIV in hippocampal culture neurons and cells were harvested at 15–18 DIV, in parallel with the time of recordings. Quantitative PCR was used to confirm the deletion of Mef2a/d (*p<0.05). Relative mRNA expression of Mef2a/d was normalized to GAPDH (GFP, n=6; Cre, n=6). Data is shown as mean ± SEM.