Figure 2. Progressive acquisition of CD200R correlates with effector cytokine secretion.

Peripheral LN cells from BALB/c mice and 4-get mice were analysed ex vivo (Naive) or after culture for 7d in transient TCR or chronic TCR stimulation conditions, as indicated, using polarising culture conditions to promote Th2 (A) or Th1 (B) cytokine differentiation. Intracellular cytokine staining with CD200R expression is shown for TNFα, IL-2, IL-4 and IFNγ. Intracellular staining with anti-GFP was used to enhance eGFP signal in 4-get cells (control stainings for anti-GFP are shown in Figure S5B & C). Contour plots show percentage of cytokine⁺ gated CD4 cells; gates were based on unstimulated controls (Figure S3A and S3B). Data are representative of 5 independent experiments. Pairwise comparison of multifunctional cytokine loss from replicate cultures is shown in Figure S3D-F.